Composite

Part:BBa_K3052034

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)


Light-fluoresce circuit (RFP) - CS

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The variables of this two-phases can be calculated by measuring the fluoresce intensity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 5916
    Illegal NheI site found at 5939
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 7476
    Illegal XhoI site found at 405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7029
    Illegal AgeI site found at 5713
    Illegal AgeI site found at 5825
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None